TRAIL 2 - PROTOCOLSTANDARDIZATION OF ANTI-AGEING FORMULATION FOR NATURAL HAIR REJUVENATE, COLOURING & GROWTH PROMOTER

PROTOCOL

STANDARDIZATION OF ANTI-AGEING FORMULATION FOR NATURAL HAIR REJUVENATE, COLOURING & GROWTH PROMOTER

MATERIALS & METHOD

Collect, mix & standardized -quantity sufficient

1)Fresh Amla berris fruit juice extraction (Phyllanthus emblica )

2)Turmeric powder - curcumin extraction (curcuma longa)

3)Beetroot juice extraction (Beta vulgaris)

4)Algae -phycobiliprotinrs extraction

5)Tea powder extraction (Camellia sinensis)

6)Heena powder extraction (Lawsonia inermis)

7) Reetha and satritha extract (Indian Soapberry)

8) Bhringraj extract (False daisy)

9) Piper Betle ext

10) Crocus SatiVus extract

11) EDTA 5%

12) 0.8% PHENOPTHELIN INDICATOR

13) 10% Polyacrylamide is a synthetic polymer produced through the free-radical polymerizationof acrylamide monomers or a combination of acrylamide and acrylic acid monomers often with a cross-linking agent like N,N′-methylenebis(acrylamide) to form a gel or network.

Blend with:

Carbonic 2.0% and Iron oxide 5.0%


Stirred for 520 speed using stirrer apparatus for 4 hours

CENTRIFUGED AT 3000 RPM IN CENTRIFUSE appratus, FILTER WITH WHATMAN FILTER PAPER .

Collect the filtrate and homonized the residue obtained above filter paper

HOMOGINISED THE ELUTION USING HOMONIZER FOR SUITABLE TIME

  1. Ninhydrin reagent solution 2% w/v in Aloevera gel, Rose water & ethanol ( should be validated)

Ninhydrin is a versatile chemical reagent primarily used

to detect primary amines, especially amino acids

, by reacting with them to produce a distinctive purple color known as Ruhemann's purple

2. Silica Gel 12%

3 Aloevera barbadensis miller 30%

CONCLUSION:

SEMI NATURAL FORMULATION USED FOR COLORING, NOURISHING AND GROWTH OF COLOURED AND NON COLOURED HAIR .

ITS GIVE SILKY, SHINY, CONDISNER AND HEALTHY HAIR

HOW TO USE

APPLY THE FORMULATION BEFORE SLEEPING AT OVER NIGHT AND WASH THE HAIR AT MORNING WHILE BATHING

APPLY ONCE IN A MONTH FOR NON COLORED HAIR AND APPLY ONCE IN A WEEK FOR COLORED HAIR

Reference

Gokhale and kokate publication book for pharmacognosy

Nirali and consine nirali prakashan book for pharmacognosy

https://www.sciencedirect.com/science/article/pii/B9780124095472001906

2019, Encyclopedia of Analytical Science (Third Edition)Chris Lennard

MARS- MAHATMA RAKESH SINGH

Mail: rakeshmahatmasingh@gmail.com

Contact: +919428013281

INDIA

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