Anti-Hypertensive & Anti-HyperLipidemic activity of Prosopis Cineraria leaves extract on Exprimental Model

 Anti-Hypertensive & Anti-HyperLipidemic activity of Prosopis Cineraria leaves extract on Pharmacological Model

Prosopis cineraria is a species of flowering tree in the pea family, Fabaceae.

Common Name :Khejri Tree, Jand, Shemi, Shami

Medicinal Use:

Prosopis Cineraria plant produces gum, which is obtained during May and June

The bark of the tree is dry, acrid, bitter with a sharp taste; cooling anthelmintic; tonic, cures leprosy, dysentery, bronchitis, asthma, leukoderma, hemorrhoids, and muscle tremors

The smoke of the leaves is good for eye troubles

Note: Please consult with the specialist first before using

Material and Methods

Preparation of Plant Extract

Hydro-methanolic (30:70) was used as solvent for the

extraction of leaves of P.Cineraria.

The plant materials were

dissolved in extraction solvent at a ratio of 1:10 (wt/vol) and

placed for 72 hours at room temperature in a shaker.

Then, the

mixture was filtered using Whatman filter paper No. 1.

The

extraction was repeated 3 times and the filtrate was collected in

a beaker.

Then the filtered extract was concentrated using

rotary evaporator at 40 C under vacuum.10 mttor

Then dry extract

was stored in air tight polythene bags until the trial was run.

Experimental Design

Albino rats (n ¼ 30) were used as experimental animals in this

study weighing about 130 + 20.5 g of 10 to 12 weeks of age.

All rats were given a standard diet and water ad libitum and

before start the trial, rats were acclimatized to handlers for

3 days. This study was approved from ethical review committee The animals were divided randomly, made 5 groups

having 6 rats (n ¼ 6) in each group.

First group G1 (normal

control) received normal diet and water ad libitum, group G2

(positive control) received only 8% sodium chloride (NaCl)

added diet (high salt diet), and group G3 was given allopathic

drug atenolol (standard drug) orally at dosage 50 mg/kg body

weight daily along high salt diet. Groups G4 and G5 were given

methanolic plant extract with dosage as low dose (100 mg/kg

body weight) and high dose (200 mg/kg body weight) daily

along with high salt diet for 4 weeks, respectively.

Measurement of Blood Pressure

Tail-cuff method is noninvasive method used to measure systolic and diastolic blood pressure, heart rate, and temperature.

Before blood pressure measurements, rats were kept in scanner

for 30 minutes to warm them. Blood pressure was measured at

the 0, 7, 15, and 30 days.

Blood Biochemical Parameters and Histopathological

Studies

At the end of fourth week samples of blood were collected in

clot activator vials by cardiac puncture. The serum was separated by centrifugation at 3000 rpm for 10 minutes. Serum

biochemical analysis including total protein was measured by

Biuret method, albumin by bromocresol green method11 and

Serum globulin was determined using the formula given below

Serum globulin ¼ Serum total protein serum albumin

.Albumin/globulin ratio was calculated by dividing the albumin concentration to globulin concentration.

Cholesterol in the

sample was determined quantitatively by cholesterol oxidase

method using the commercially available kit spectrophotometrically.

Triglyceride was measured using enzymatic kit

method following the protocol of the study by Shephard and

Whiting with the help of spectrophotometer.

High-density

lipoprotein (HDL)–Cholesterol was determined calorimetrically using the procedure described by Friedewald et al,

low-density lipoprotein (LDL)–Cholesterol and very lowdensity lipoprotein (VLDL)–Cholesterol were calculated using

the formulas described by Friedewald et al, given below:

LDL cholesterol mg=dL¼ Total cholesterol TG=5Þ

HDL cholesterol:

Liver and kidney tissues were collected from the sacrifice

rats for histopathological examination. The tissue sections were

processed to embed in paraffin wax and the slides were prepared by staining with hematoxylin and eosin stain after

microtomy and observed under light microscope to note any

type of morphological changes in tissues as described in the

study by Spencer et al.

Statistical Analysis

All results were presented as mean + standard error of mean.

The obtained data were analyzed statistically using 1-way analysis of variance and Tukey test were used to determine pairwise

comparison using statistical Software SPSS 23 (Trial version).

P value less than 05 was representing the significant difference

between groups.

#pharmascienceprotocol

#keshbluebite

#MARSmahatmaRakeshSingh

#keshAntiHypertensive

#KeshAntiHyperlipidemic

#kesh ProsopisCineraria

#RndDSpectra

#Rakfesh

#SchizophreniaUnzippedLiveNdPharmaProtocols