Antidepressant activity of NameImpatiens glandulifera leaves extracts on mice model

 Antidepressant activity of NameImpatiens glandulifera leaves extracts in mice model

HIMALAYAN BALSAM

The Country Land and Business Association says the weeds - such as Giant Hogweed, Himalayan Balsam, Japanese Knotweed and Floating Pennywort can be dangerous to humans, animals and other plants.

Common NamesHimalayan Balsam, Policemans Helmet, Bobby Tops, Copper Tops

Scientific NameImpatiens glandulifera

Season Start -Mar Season

End -Nov

PHARMACOLICAL USES

AN EXTRACTION OF PLANT IS USED IN HMEOPATHY FOR THE TREATMENT OF ANXIETY

METHODS

Antidepressant activity was studied in albino mice using forced swimming test (FST), tail suspension test (TST) and shock induced depression (SID). Imipramine (10mg/kg), fluoxetine (30mg/kg) were used as standard drugs and NGE (10, 20, 30mg/kg) was used as test drug.

FST

The water tanks

The cylindrical tanks (30 cm height x 20 cm diameters) required for the mouse forced swim test (FST) in our laboratory are constructed of transparent Plexiglas, as this material is able to withstand the frequent movement of the tanks and accidents better than glass. The water level is 15 cm from the bottom and should be marked on the tank to ensure that the volume of water is consistent across mice. The number of tanks should ideally be at least twice as many as the number of mice being tested at a time, so that the second water tank set can be filled while the first set is in use. The dimensions of the tanks should be selected in a way that the mice will not be able to touch the bottom of the tank, either with their feet or their tails, during the swimming test. The height of the tank should be high enough to prevent the mice from escaping from the tank. Please note that the diameter of tank and the depth of water are important parameters that can be adjusted to change the behavior of mice

Video tracking allows you to detect some behaviors automatically, such as immobility, which is indicative of freezing. The three variations of mobility – immobile, mobile, and highly mobile – indicate the three most important behaviors in the forced swim test: floating, swimming, and climbing/ escaping.

There are two ways to detect this with EthoVision XT, and they can be used alongside each other. First is by tracking the center point of the animal and determining if it is immobile or not. Thresholds can be defined by the user. Second, you can use activity detection. This method might even give you more robust data as it focuses on changes in the arena from one video frame to the next.

Tail suspension test

The tail suspension test (TST) is a behavioral task for the screening of potential antidepressant agents in mice (Mahmoudi et al., 2015). This test is based on the fact that short-term, inescapable stress of suspension of animals by their tails leads to an immobile posture, and antidepressant agents typically reduce the frequency and duration of immobility (Cryan et al., 2005). Although sophisticated modes of delivery improve the quality of data in TST, basically, the procedure only requires a suspension bar and tape (Can et al., 2012b). The task is a one-step test and duration is usually 6 minutes. The TST as a behavioral task has been used in the PBM and depression mice models conducted by Xu et al. (2017) and Ando et al. (2011).

Measurement of locomotor activity test (MLAT)

The locomotor activity test needs to be performed according to the method of Carlini with minor modifications. This test is designed to measure the mobility

of mice. A group of 25 mice was divided at random into

five groups and orally administered with control, extract

or Imipramine hydrochloride 30 min before the experiment. Mice were placed in an open field apparatus composed of an arena 40 cm in diameter divided into 16

approximately equal areas. For open field observations,

each mouse was individually placed in the center of the

arena 15 h after the last treatment. Hand-operated counters were employed to score the following behavioral parameters: locomotion (number of line crossings), rearing frequencies (number of times seen standing on hind

legs), and number of defecations within 5 min. Open

field observations was made between 8 and 10 a.m

#pharmascienceprotocol

#rakfeshNameImpatiensglandulifera

#rakfeshAntidepressant

#rnddspectra

#scizophreniaunzippedlive

#Ayurvedahezards

#rakfeshbluebite

#rakfesh